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rsiV [2018-02-08 10:56:36]
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rsiV [2018-02-08 10:56:36]

anti-[SW|sigma factor] to [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV]
locus
BSU27130
pI
5.83
mw
32.72 kDa
protein length
285 aa Sequence Blast
gene length
855 bp Sequence Blast
function
control of [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV] activity
product
anti-[SW|sigma factor] to [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV]
essential
no
synonyms
yrhM
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,770,350 → 2,771,207

    The protein

    Catalyzed reaction/ biological activity

  • binds and thus inactivates [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV]

    • Effectors of protein activity

  • RsiV directly binds lysozyme [Pubmed|25275625]
  • upon exposure to lysozyme, the extracellular domain of [protein|ED1DDA187692683D9862EC0E858B80CD56C48045|RsiV] is cleaved off by [protein|CDC6971F39F3EEAAE912CB1402EE1BD64D5A12A0|SipS] and [protein|search|SipT ](between A66 and M67), subsequently the N-terminal part of the protein is subject to intramemrane proteolysis by [protein|CB50289535EA537F63BADD459BD11AA7759A6658|RasP] [Pubmed|29358498,25275625,23687273]

    • Structure

  • [PDB|5JEN] (complex with lysozyme)

    • [SW|Localization]

  • membrane [Pubmed|23687273]

    • Expression and Regulation

    Operons

    genes
    [gene|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV]-[gene|ED1DDA187692683D9862EC0E858B80CD56C48045|rsiV]-[gene|E97C7A219DB059BB634E893CC355A28324C87ADB|oatA]-[gene|FC37C7A0C0D9F50CA1D44B8D939CF06E210D4B38|yrhK]
    description
    [Pubmed|21856855]

    sigma factors

  • [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV]: sigma factor, [Pubmed|21856855], in [regulon|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV regulon]

    • regulation

  • induced by lysozyme ([protein|search|SigV]) [Pubmed|21856855]

    • additional information

  • A [protein|search|ncRNA] ([SW|RnaC]) is encoded between '[protein|search|yrhK]' and '[protein|search|yrhJ]' [PubMed|25790031]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A145 (yrhM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/145 NBRP B. subtilis, Japan]
  • BKE27130 (Δ[gene|ED1DDA187692683D9862EC0E858B80CD56C48045|rsiV]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATTGCTGTAATCTCTTAT, downstream forward: _UP4_TAAAATAAAAACATCCATCT
  • BKK27130 (Δ[gene|ED1DDA187692683D9862EC0E858B80CD56C48045|rsiV]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATTGCTGTAATCTCTTAT, downstream forward: _UP4_TAAAATAAAAACATCCATCT

    • References

  • 14993308,16274938,20817771,23687273,25275625,26399770,29358498